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Anti-inflammatory Analysis: NFkB inhibition in cells

NF-κB (Nuclear Factor kappa B), a protein complex that is involved in cellular responses to stimuli such as stress and free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. It plays a key role in regulating the immune response to infection. Suppression of NF-κB limits the production of proinflammatory gene expression and reduces the level of inflammation, therefore NF-κB has been studied as a biomarker for inflammation, and inhibition of NF-κB has been used as an indicator for anti-inflammatory potential. A classic example of anti-inflammatory material via NF-κB inhibition are natural compounds such as curcumin and green tea  polyphenols that have been demonstrated to inhibit inflammation via NF-κB inhibition.

In this analysis, human cells are first treated with or without a test material to allow for natural absorption of the material into the cells. Then, a proinflammatory cytokine is introduced to the human cells to mimic cellular stimulation which would normally induce NF-κB activation leading to inflammation. If an NFkB inhibiting material presents in the cellular environment, the material inhibits NFkB activation and the degree of inhibition can be monitored via NFkB expression. NFkB expression level of the human cells, treated with and without the test material, under the stressed condition are therefore monitored and compared to assess the NFkB inhibition effect of a material.

We provide two models in NFkB analysis:

  • Cellular Anti-inflamamtory Analysis: NFkB
  • Enzymatic Anti-inflamamtory Analysis: NFkB