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Lipooxygenase (LOX) Inhibition Assay

Lipoxygenases (LOXs) are a family of nonheme iron-containing dioxygenases enzymes distributed in animals, plants, and fungi. These enzymes catalyze distinct cellular reactions and produce fatty acid hydroperoxides throughout the reaction processes. These fatty acid hydroperoxides products have been identified as mediators of a series of inflammatory diseases including rheumatoid arthritis, inflammatory bowel disease, atherosclerosis and certain types of cancer. Therefore LOX inhibitors have been discovered as beneficial to inflammation treatment. Another significance in LOX inhibition is that LOX 5 inhibitors have been identified to potentially counteract the gastric damage associated with the COX-1 inhibitors. This makes LOX 5 inhibitors potential side effect removers for many COX-1 inhibitor anti-inflammatory drugs including aspirin.

Through Lipooxygenase (LOX) Inhibition investigation we determine the inhibition potential of a material on LOXs activity via enzymatic analysis. Inhibition of two main LOX enzymes can be investigated via these analyses:

  • LOX-5
  • LOX-15