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TOTAL PHENOL
Total phenol assay in Brunswick Labs was adopted from Folin-Ciocalteau
method.
Folin-Ciocalteau method
was initially intended for the analysis of proteins taking advantage of the reagent’s
activity toward protein tyrosine (containing a phenol group) residue. Many years
later, Singleton and co-workers extended this assay to the analysis of total phenols
in wine. The FC-based assay gained popularity. The FC actually measures a sample’s
reducing capacity, but this is not reflected in the name “total phenolic assay?
Despite the undefined chemical nature of FC, the total phenols assay by FC is convenient,
simple, and reproducible. As a result, a large body of data has been accumulated,
and it has become a routine assay in studying phenolic antioxidants.
Learn more:
Total Phenol Analysis: Automation and Comparison with Manual Methods
